Medimeas Instruments is a leading manufacturer and exporter of microtomes based in India. We manufacture a complete range of Microtomes: Manual Rotary Microtomes, Semi Automatic Microtomes, Fully Automatic Microtomes, Manual Cryostat Microtomes and Semi Automatic Cryostat Microtomes

Our organization is ISO 9001:2008, ISO 13485:2003 certified & our microtomes are CE approved. Our company was established in 1975 and over the years we have been introducing innovative microtomes to the world market

What we do

We manufacture complete range of Rotary Microtomes, Semi Automatic Microtomes, Fully Automatic Microtomes, Cryostat Microtomes for routine histology procedures 

MRM-RM MANUAL ROTARY MICROTOME

 Product VIDEO

 MRM-ST SEMI AUTOMATIC MICROTOME

MRM-AT FULLY AUTOMATIC MICROTOME

MCM-AT FULLY AUTOMATIC CRYOSTAT MICROTOME

Write to us at abhinav@medimeas.com for a product catalogue

Cell:+919215699665

What is a Microtome

A microtome is a sectioning instrument that allows for the cutting of extremely thin slices of material, known as sections so as to view them under a microscope. A typical microtome consists of a stationary blade and a moving feed. The up and down movement of the feed on the blade cuts fine sections of paraffin embedded tissues.

A Retracting mechanism is a standard feature in a Microtome, in which the feed moves back from the blade during the return stroke, this is to prevent damage to the tissue during the non-cutting return stroke.

The Anti-roll plate prevents rolling/curling of the sections and allows sections to form a ribbon so that minimum specimen handling is required which in turns improves the quality of the sections. It is usually found in Cryostat Microtomes, where curling is an issue during frozen sectioning.

A Coarse feed wheel in a manual microtome is used to adjust the specimen feed at the right position with respect to the knife. The same function is motorised in a semi automatic microtome.

CRYOSAT MICROTOME

In a cryostat microtome, the whole microtome assembly is placed inside a closed chamber with temperature as low as -40 deg C.

New Generation Cryostat Microtomes have separate cooling systems for the chamber and the freeze shelf. A regular compressor for the chamber and an independent Peltier Unit for the freeze shelf. This allows for rapid specimen freezing on the freeze shelf prior to sectioning.

Double compressor Cryostat Microtomes can achieve low temperatures, as low as -50 deg Celsius, in just 60 mins.

Other standard features in a Cryostat Microtome includes a special frost free glass for clear visibilty during sectioning, a built in illumination, UV irradiation of the chamber, option of auto/manual defrosting, a condensate collection bottle for removal of condensed liquids.

In Semi Automatic Cryostat Microtomes, the specimen feed is accurately controlled through a precise stepper motor. The speed at which specimen advances is usually a single optimised fixed speed but some manufactureres do offer two speeds; low and high speed.

In a Fully Automatic Cryostat Microtome, the sectioning is controlled by a motor i.e. the vertical movement of the specimen against the blade is controlled by a stepper motor. This eliminates the need to turn the handwheel. The speed of sectioning can be continuously adjustable or can be increased in steps, usually three: low, medium and high.

The other standard feature in a Cryostat Microtome is the automatic and manual defrosting function which is essential to keep work area frost free. The fluids from the defrosting operation are collected in a bottle which is conveniently placed at the front of the equipment. The bottle can be easily removed, drained and then fitted back in place.

 The Cryostat Microtome is fitted with special glass that prevents build up of frost on its surface. This along wih the illumination light provides a clear view of the inside of the chamber.

The Freeze shelf inside a Cryostat Microtome chamber holds the tissues to be frozen. The tissue can be fixed on to specimen head using different techniques. A specially designed annular specimen heads are used for this purpose. The tissue samples are placed on these specimen heads and embedding medium is applied on top. The cold temperature of the freeze shelf freezes the tissue which is now ready to be sectioned.

A Cryostat Microtome is better than a Microtome with cooling attachments where only specimen holder and the knife is cooled. These advantages are as follows:

1. The dry atmosphere inside the Cryostat Microtome prevents the formation of ice crystals on the specimen which can cause a change in chemical composition of the Tissue
2. As the Microtome is isolated from the atmosphere, no condensation occurs on the knife surface, this prevents the formation of Rime.
3. Cryostat Microtomes are usually fitted with Anti roll device which prevents the curling of sections, a common issue in Frozen Sectioning. On the other hand Microtomes with cooling attachments cannot be fitted with Anti-Roll Device
4. Uniform temperature conditions around the object and the knife give better cutting results.